A home-made heating accessory for UV-Vis cells was developed and tested in both DLS and UV-Vis in-situ measurements. The device is designed to be placed in a measurement chamber of the two-beam spectrophotometer (SHIMADZU UV-2600). It is made of aluminum, and contains four ceramic resistors for the simultaneous uniform heating of two standard UV-Vis cells. The outer thermal emittance is minimized by a wooden thermo-insulating shield. The accessory is painted matte black to minimize beam dispersion of the spectrophotometer. DC power supply allows to maintain temperature of the UV-Vis cells in the interval from 30 to 120 °C. The optical probe of the DLS can be immersed into heated liquid sample in a standard UV-Vis cell. The reaction of the formation of gold nanoparticles in heating accessory was in-situ monitored by simultaneous measurements with SHIMADZU UV-2600 and Microtrac Nano-flex particle size analyzer as an example.

Keywords: Heating cell, DLS, Uv-vis, in-situ analysis,gold nanoparticles